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10. | | PEREIRA, A. A.; OLIVEIRA, A. C. B. de. Cultivares. In: SAKIYAMA, N. S.; PRIETO, H. E.; MARTINEZ, H. E. P.; TOMAZ, M. A.; BORÉM, A. (Org.). Café Arábica: do plantio à colheita. 1. ed. Viçosa: Editora UFV, 2015, v.1, p. 24-45. Biblioteca(s): Embrapa Café. |
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12. | | MENNA, P.; PEREIRA, A. A.; BANGEL, E. V.; HUNGRIA, M. Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. Symbiosis, Philadelphia, v. 48, n. 1-3, p. 120-130, Feb. 2009. Biblioteca(s): Embrapa Soja. |
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14. | | BARBOSA, I. de P.; OLIVEIRA, A. C. B. de; ROSADO, R. D. S.; SAKIYAMA, N. S.; CRUZ, C. D.; PEREIRA, A. A.; PEREIRA, A. A. Sensory quality of Coffea arabica L. genotypes influenced by postharvest processing. Crop Breeding and Applied Biotechnology, v. 19, n. 4, p. 428-435, 2019. Biblioteca(s): Embrapa Café. |
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20. | | NASCIMENTO, F. S. S. do; SIVIERO, A.; CARVALHO, J. O M.; PEREIRA, A. A. A. Caracterização botânica de genótipos de mandioca (Manihot esculenta) em Ouro Preto do Oeste, RO. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 4 p. 1 CD-ROM. Biblioteca(s): Embrapa Acre. |
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Registros recuperados : 199 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
27/07/2009 |
Data da última atualização: |
04/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
MENNA, P.; PEREIRA, A. A.; BANGEL, E. V.; HUNGRIA, M. |
Afiliação: |
PÂMELA MENNA, UEL/CNPq-MCT; ALAN ALVES PEREIRA, CNPq-MCT; ELIANE VILLAMIL BANGEL, FEPAGRO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Symbiosis, Philadelphia, v. 48, n. 1-3, p. 120-130, Feb. 2009. |
ISSN: |
0334-5114 |
DOI: |
10.1007/BF03179991 |
Idioma: |
Inglês |
Conteúdo: |
With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies. |
Thesagro: |
Bactéria; Fixação de Nitrogênio; Inoculante; Taxonomia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01846naa a2200229 a 4500 001 1471352 005 2017-08-04 008 2009 bl uuuu u00u1 u #d 022 $a0334-5114 024 7 $a10.1007/BF03179991$2DOI 100 1 $aMENNA, P. 245 $aRep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool. 260 $c2009 520 $aWith more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N2 fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach ? 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups ? might expedite rhizobial diversity studies. 650 $aBactéria 650 $aFixação de Nitrogênio 650 $aInoculante 650 $aTaxonomia 700 1 $aPEREIRA, A. A. 700 1 $aBANGEL, E. V. 700 1 $aHUNGRIA, M. 773 $tSymbiosis, Philadelphia$gv. 48, n. 1-3, p. 120-130, Feb. 2009.
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